M.Sc. Bio-Chemistry (PU-CET P.G) 2017 Panjab University Entrance Exam With Answers

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36.

Digestion of a 5Kb linear DNA fragment with EcoRI generates two fragments of 2 Kb and 3 Kb, while digestion of the same molecule with HindIII yields three fragments of 0.7 Kb, 3.5 Kb and 0.8 Kb. When the same DNA is digested with both the enzymes, it yields fragments of 0.7 Kb, 1.3 Kb, 2.2 Kb and 0.8 Kb. The right sequence of restriction sites in the DNA fragment is

A: One EcoRI site in between two HindIII sites
B: One HindIII site in between two EcoRI sites
C: Two HindIII sites followed by only one EcoRI site
D: One EcoRI site followed by two HindIII sites.

The answer is: A

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