Bio-Chemistry (M.Phil./Ph.D.) 2016 Panjab University Entrance Exam With Answers

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43.

A DNA molecule when digested with different restriction enzymes resulted in following fragments.

BamH1- 12 kb, 20 kb
Xho1 -18 kb, 14 kb
BamH1+Xho1 -12kb,14kb,6kb
(i) DNA molecule is linear with one site each for BamHI and Xhol.
(ii) DNA molecule is circular with one site each for BamHI and Xhol.
(iii) Total length of DNA molecule is 32 kb.
(iv) BamHI is blunt while Xhol is a sticky cutter enzyme.
Which statement is true ?
A: (i) and (iii)
B: (ii) and (iii)
C: (i) and (iv)
D: (ii) and (iv)

The answer is: A

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