1.
During each cycle of PCR the reaction mixture is transferred between three temperatures. All the following statements are true for these temperatures, except;
A:
The denaturation temperature helps to release the single stranded DNA to act as templates
B:
The hybridization temperature facilitates primers to attach to the templates.
C:
Annealing temperature is the one at which DNA synthesis occurs.
D:
The extension temperature is usually set just below the optimum temperature of Taq polymerase.
The answer is:
C
