34.
A novel sea organism was identified giving blue luminiscense. For observing the true expression of the gene responsible for this character which of the following strategies will you follow ?
A:
Isolate the genomic DNA from the organism and directly injecting to bacteria, screen colonies for bioluminescence.
B:
Isolate the genomic DNA from the organism, treat with DNAsel, followed by restriction endonuclease treatments and transform into eukaryotic cell such as yeast, screen colonies for bioluminescence.
C:
Isolate the mRNA from the organism, reverse transcribe and generate cDNA, transform the virus and screen colonies for bioluminescence.
D:
Isolate the mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast screen, colonies for bioluminescence.
The answer is:
D
