6.
A reporter gene construct consisted of NFAT responsive promoter linked to a green fluorescence protein (GEP) gene fragment and the construct was transfected to animal cells in culture. Treatment of these cells with X compound led to expression of GEP and the cells looked green. Which of the following led to such an effect?
A:
Compound X signals to hydrolyze the NFAT promoter region to release GFP for giving green colour
B:
Compound X acted as antisense to blocked NFAT expression hence only GFP gets expressed and gives green colour
C:
Compound X signals to synthesize aromatic amino acid rich protein that gave overall green colour to the cells
D:
Compound X signals to translocate cells native NFAT to Nucleus leading to expression of GEP to given green colour
The answer is:
D
