You are asked to perform the PCR reaction using Pfu polymerase and not with Taq polymerase Why do you think this modification was suggested ?

Biotechnology Engineering (M.Phil./Ph.D.) 2017 Panjab University Entrance Exam With Answers

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15.

A: Pfu polymerase temperature optima is more than 100 C.

B: Taq polymerase has strong proofreading and does not allow completion of PCR cycles

C: Pfu lacks the proof reading so that amplification is quick

D: Taq polymerase does not have proofreading activity

The answer is: D

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